Transmission Electron Microscope Facility

Genotype to phenotype
Project contact
Cameron Chalmers
Consultancy Associate
Cameron.Chalmers@ei.ed.ac.uk

Transmission Electron Microscope helps research to turbo-charge plants' CO2 capture

The McCormick group at the University of Edinburgh is using synthetic biology to try and turbo-charge photosynthesis in plants using components from green algae.

To capture CO2 during photosynthesis, plants use an enzyme called Rubisco that operates so poorly that it actually limits yields in most crop plants. In contrast, many green algae have developed a specialised CO2-concentrating mechanism (CCM) in which Rubisco is aggregated into a unique phase-separated compartment inside the chloroplast called the pyrenoid.

The pyrenoid was one of the first subcellular organelles visualised by early microscopes over 100 years ago. Now, efforts to engineer pyrenoid-based CCMs into plants to improve their photosynthesis efficiencies are making use of more advanced microscopy techniques.

Nicky Atkinson, a postdoc from the McCormick group, has been working closely with Steve Mitchell from the School of Biological Sciences’ Transmission Electron Microscope (TEM) facility to develop a pipeline for visualising plant tissue containing a synthetic proto-pyrenoid.

Having characterised the proto-pyrenoid as far as possible by confocal microscopy, TEM became essential to track the introduction of additional components, including a starch sheath and a traversing thylakoid membrane, which will be required to make the CCM fully functional.

Thanks to Steve’s expertise, a protocol for fixing and embedding tissue was rapidly established. Nicky has been able to screen various iterations of the proto-pyrenoid over the course of five years and has employed immunogold labelling of TEM sections in order to visualise the localisation of introduced components.

Steve has also now trained Nicky in the use of the ultramicrotome for creating her own sections, imparting high-level skills that will be fundamental to the McCormick group’s development of plants with enhanced CO2 fixation in future years.

Transmission Electron Microscope Facility

The Transmission Electron Microscope Facility

Specialisms

  • Agri-tech, agri-food and plant biology
  • Food and drink
  • Healthcare and disease
  • Life sciences and industrial biotechnology
  • Pharmaceuticals and medical biotechnology


Technologies available

The TEM facility offers a variety of preparation and imaging services for room-temperature samples, including plastic sections, negative-stain and shadowed materials. Training can be provided.

  • TEM processing to block
  • SEM processing to stub
  • Critical point drying
  • Semi-thin sectioning
  • Ultra-thin sectioning
  • Sputter coating
  • Negative staining
  • Carbon coating
  • Provision of grids


Equipment available

Our wide range of equipment includes:

  • Jeol JEM1400 Plus Transmission Electron Microscope with OneView Camera
  • Gold Palladium Sputter Coater
  • Quorum Glow Discharger
  • Leica ACE-600 Rotary Shadower
  • Leica Ultracut UCT Ultramicrotome
  • Edwards Vacuum Coater


More details

The Transmission Electron Microscope Facility

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