Bacterial strains for antibiotic drug discovery
This technology consists of 12 K. pneumoniae strains that which have been genetically engineered to carry mutations affecting key cell functions known to be implicated in the development of drug resistance including antimicrobial efflux, virulence, transcription factor regulation and membrane stability. These are useful set of strains for use in antibiotic drug discovery and screening.
Useful as a model of various Gram-negative bacteria
Drug molecule screening and resistance mechanism profiling.
Early stage laboratory Data
Licensing and/or collaboration
K. pneumoniae is classed by WHO as posing a severe threat to human health and is responsible for a variety of common hospital acquired infections. Klebsiella pneumoniae is highly drug resistant and is rapidly becoming the cause of untreatable infections globally. Understanding the molecular tricks the bacteria uses to become resistant is likely to be key to identifying new effective antibiotics that avoid these resistance mechanisms.
A series of K. pneumoniae strains with key phenotypes associated with resistance, including enhanced efflux, virulence and outer-membrane stability, which can be used to characterise and profile the activity of new antibiotic candidates to identify those able to circumvent these resistance mechanisms.
Comprehensive genetic and phenotypic data on each of both wildtype and mutant strains and dose response data to selected antibiotics including fluroquinilones and tetracyclines.
- A different target to investigate and screen against
- Useful as a model system for other Gram-negative bacteria
- Ability to screen new antimicrobials to assess if they would be susceptible to known pathways of drug-resistance
De Majumdar S et al. Elucidation of the RamA Regulon in Klebsiella pneumoniae Reveals a Role in LPS Regulation. PLoS Pathogens 2015.
Veleba M et al. Characterization of RarA, a Novel AraC Family Multidrug Resistance Regulator in Klebsiella pneumoniae. Antimicrobial Agents and Chemotherapy 2012.